Sequencing-based approaches in plant molecular biology

Elucidation of molecular mechanisms associated with stress responses in stressed plants through large-scale data analysis.

• Differential gene/microRNA expression analyses of RNA-Seq and miR-Seq data

• Development of functional markers

• Construction of protein–protein interaction networks

• Identification of candidate genes that may play roles in stress responses

Plant tissue culture applications

Micropropagation and plant regeneration of economically important plant species using tissue culture methods, and their application in Agrobacterium-mediated gene transfer studies

• Development of suitable tissue culture methods for economically important plant species such as saffron, including micropropagation and plant regeneration

• Simulation of plant stress environments using tissue culture methods - Application of nanoparticles using plant tissue culture methods

• Agrobacterium-mediated CRISPR and plasmid transfer studies involving transgenes

Morphological, Physiological, and Biochemical Analyses

Elucidation of the cellular responses of plants to biotic and abiotic stress factors using morphological, physiological, and biochemical methods.

• Examination of growth and development parameters in plants under stress conditions

• Examination of physiological parameters in plants

• Measurement of biochemical activity in the cellular signaling pathway and determination of antioxidant enzyme activities.

The use of nanotechnology

The production of nanoparticles via green synthesis, their characterization, and the identification of their roles in plant responses to stress.

• The production of nanoparticles via green synthesis and the performance of characterization analyses

• The application of nanoparticles as a restorative agent in plants exposed to stress

• Elucidating the roles of nanoparticles in plant development processes

Genetic diversity analyses and mutation breeding

Conducting genetic diversity analyses relevant to variety development studies to identify potential parental lines. Additionally, using mutagens to generate new plant varieties and expand the gene pool.

• Conducting genetic diversity analyses in plant populations using various marker systems

• Conducting molecular characterization studies in plant populations

• Generating new variations through mutagen applications. Conducting molecular and morphological analyses of these variations